種屬

人

別稱

Gp2d; Gp2D; GP2D

組織來源

結(jié)腸

疾病

結(jié)腸癌

傳代比例/細(xì)胞消化

1:2傳代,消化2-3分鐘。

完全培養(yǎng)基配置

DMEM培養(yǎng)基；10%胎牛血清；1%雙抗

簡介

GP2d has been established from the same adenocarcinoma as GP5d (ECACC catalogue no. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF alpha or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.

形態(tài)

上皮細(xì)胞樣

生長特征

    貼壁生長

STR

Amelogenin X  CSF1PO 9,12 D2S1338 18 D3S1358 16,18 D5S818 11,12 D7S820 9,11 D8S1179 10,12

D13S317 8,12 D16S539 11,13 D18S51 12,13 D19S433 12,13 D21S11 29,30 FGA 23 PentaD 12,13

PentaE 7,10 TH01 7,9.3 TPOX 8,11 vWA 17,18 D6S1043 10,17 D12S391 19,22 D2S441 10,11

倍增時(shí)間

48-60h

培養(yǎng)條件

氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。

凍存條件

凍存液：90%FBS，DMSO 10%,或使用非程序凍存液：官網(wǎng)貨號(hào)JY-H040

保藏機(jī)構(gòu)  

ECACC; 95090714

產(chǎn)品使用

僅限于科學(xué)研究，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。